BERTHELIN Clothilde
Maître de Conférences
PHARE
CAMPUS I, CAEN
clothilde.berthelin@unicaen.fr
+33 2 31 56 51 14

Thèmes de recherche

Mots clés :

Vésicules extracellulaires-Mollusques -Bivalves-Changement climatique-Acclimatation-Energie-Reproduction-Hypoxie

Mes recherches portent sur la physiologie des mollusques bivalves avec un modèle d’étude principal, une espèce emblématique de la côte normande, l’huître Magallana gigas. Je m’intéresse aux processus métaboliques des bivalves impactés par les conditions environnementales, dans un contexte de changement climatique. Mes recherches actuelles portent sur la régulation des réponses aux changements environnementaux (hypoxie, température, conditions trophiques) chez les huîtres, plus précisément aux processus médiés par les vésicules extracellulaires.

Méthodologies employées :
Méthodologies d’isolement et caractérisation des EVs, microscopies optique, confocal et électronique, techniques d’enrichissement cellulaire, cytométrie en flux, analyse cellulaire basée sur l’impédance et primo cultures de cellules d’huître.

Expertise scientifique :

  • Isolement et caractérisation des vésicules extracellulaires chez l’huître
  • Connaissance approfondie de l’anatomie, des organes et des tissus des mollusques bivalves, étudiés en microscopie et avec des approches en immunofluorescence
  • Analyse des cellules souches et des proliférations cellulaires dans la gonade de M. gigas
  • Etude de la gamétogenèse chez diverses espèces de bivalves (M. gigas, Corbicula sp., Ruditapes decussatus) par histologie qualitative et histo-imagerie quantitative

Enseignements et diffusions des connaissances

  • Enseignements en biologie, analyse des produits alimentaires, bases d’agronomie (filière produits aquatiques).
  • Conception et animation d’ateliers lors de la Fête de la Science
  • Conférences grand public (Fête de la Science, université inter-âges)

Responsabilités administratives

  • Responsable des parcours intégrés ingénieur en licence Science Vie et Sciences pour la Santé, Université de Caen Normandie
  • Responsable de la filière Produits aquatique au sein de l’ESIX AgroA, école d’ingénieurs agroalimentaires de l’université de Caen Normandie.
  • Responsable de la deuxième année du cycle ingénieur agro-alimentaire et des projets tutorés de première année.
  • Membre du conseil de laboratoire MERSEA
  • Membre du conseil d’école de l’ESIX Normandie
  • Membre du conseil du département agro-alimentaire de l’ESIX Normandie

Galerie d'images

Article dans une revue

2026

ref_biblio
Sauger, C., Quinquis, J., Kellner, K., Berthelin, C., Lepoittevin, M., Elie, N., & Dubroca, L. (2026, Feb). Comparing Macroscopic and Quantitative Histological Methods to Determine Sexual Maturity in the Female European Plaice. Animals, 16 (519), 1-18. <10.3390/ani16030519>. <hal-05497774>.

2024

ref_biblio
Etoundi, E., Vastrade, M., Berthelin, C., Kellner, K., Fafin-lefèvre, M., & Van Doninck, K. (2024). Transition from sexuality to androgenesis through a meiotic modification during spermatogenesis in freshwater Corbicula clams. PLoS ONE, 19 (11), e0313753. <10.1371/journal.pone.0313753>. <hal-04964263>. PDF
ref_biblio
Sol Dourdin, T., Berthelin, C., Guyomard, K., Morin, A., Morandi, N., Elie, N., Villain-naud, N., Rivière, G., & Sussarellu, R. (2024, Aug). The Pacific oyster reproduction is affected by early-life exposure to environmental pesticide mixture: A multigenerational study. Science of the Total Environment, 937, 173569 (10). <10.1016/j.scitotenv.2024.173569>. <hal-04669241>.

2023

ref_biblio
Sauger, C., Quinquis, J., Berthelin, C., Lepoittevin, M., Elie, N., Dubroca, L., & Kellner, K. (2023). A Quantitative Histologic Analysis of Oogenesis in the Flatfish Species Pleuronectes platessa as a Tool for Fisheries Management. Animals, 13 (15), 2506. <10.3390/ani13152506>. <hal-04204143>. PDF

2022

ref_biblio
Sussarellu, R., Chouvelon, T., Aminot, Y., Couteau, J., Loppion, G., Dégremont, L., Lamy, J.-B., Akcha, F., Rouxel, J., Berthelin, C., Briaudeau, T., Izagirre, U., Mauffret, A., Grouhel, A., & Burgeot, T. (2022, Jan). Differences in chemical contaminants bioaccumulation and ecotoxicology biomarkers in Mytilus edulis and Mytilus galloprovincialis and their hybrids. Environmental Pollution, 292 (Part. A), 118328 (16). <10.1016/j.envpol.2021.118328>. <hal-04203640>. PDF

2020

ref_biblio
Sauger, C., Quinquis, J., Kellner, K., Berthelin, C.H., Lepoittevin, M., Elie, N., & Dubroca, L. (2020, Dec). A macroscopic and stereological imaging dataset of Pleuronectes platessa ovaries. Scientific Data, 7, 165. <10.1038/s41597-020-0505-8>. <hal-03331944>. PDF

2019

ref_biblio
Cherif-feildel, M., Berthelin, C.H., Rivière, G., Favrel, P., & Kellner, K. (2019, Feb). Data for evolutive analysis of insulin related peptides in bilaterian species. Data in Brief, 22, 546-550. <10.1016/j.dib.2018.12.050>. <hal-02143349>.
ref_biblio
Cherif-feildel, M., Berthelin, C.H., Adeline, B., Favrel, P., Kellner, K., & Rivière, G. (2019, Jan). Molecular evolution and functional characterisation of insulin related peptides in molluscs: Contributions of Crassostrea gigas genomic and transcriptomic-wide screening. General and Comparative Endocrinology, 271, 15-29. <10.1016/j.ygcen.2018.10.019>. <hal-02142617>.
ref_biblio
Cherif-feildel, M., Kellner, K., Goux, D., Elie, N., Adeline, B., Lelong, C., & Berthelin, C.H. (2019, May). Morphological and molecular criteria allow the identification of putative germ stem cells in a lophotrochozoan, the Pacific oyster Crassostrea gigas. Histochemistry and Cell Biology, 151 (5), 419-433. <10.1007/s00418-018-1740-3>. <hal-02142626>. PDF
ref_biblio
Sauger, C., Quinquis, J., Dubroca, L., Parrad, S., Kellner, K., & Berthelin, C. (2019). Sample collection protocol for the extraction of female gonads in the megrim (Lepidorhombus spp) for maturity staging through histology. ARCHIMER Archive institutionnelle de l'IFREMER. <10.13155/70724>. <hal-04977959>.

2016

ref_biblio
Barranger, A., Berthelin, C., Rouxel, J., Adeline, B., Benabdelmouna, A., Burgeot, T., & Akcha, F. (2016). Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas. Comparative Biochemistry and Physiology - Part C: Comparative Pharmacology, 180, 23-30. <10.1016/j.cbpc.2015.11.002>. <hal-04978003>.
ref_biblio
Boonmee, A., Berthelin, C.H., Kingtong, S., Pauletto, M., Bernay, B., Adeline, B., Suquet, M., Sourdaine, P., & Kellner, K. (2016, Nov). Differential protein expression during sperm maturation and capacitation in an hermaphroditic bivalve, Pecten maximus (Linnaeus, 1758). Journal of Molluscan Studies, 82 (4), 575-584. <10.1093/mollus/eyw028>. <hal-01483184>.

2013

ref_biblio
Kingtong, S., Kellner, K., Bernay, B., Goux, D., Sourdaine, P., & Berthelin, C.H. (2013, Apr). Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas. Journal of Proteomics, 82, 81-91. <10.1016/j.jprot.2013.02.009>. <hal-02296503>.

2011

ref_biblio
Berthelin, C., Hanquet, A.-C., Jouaux, A., Michel, M., & Kellner, K. (2011). A sodium co-transporter (SGLT) for glucose transport into Crassostrea gigas vesicular cells: Impact of alimentation on its expression. Aquaculture. <hal-04977993>.
ref_biblio
Jouaux, A., Franco, A., Heude-berthelin, C., Sourdaine, P., Blin, J.L., Mathieu, M., Kellner, K., & Berthelin, C.H. (2011, Dec). Identification of Ras, Pten and p70S6K homologs in the Pacific oyster Crassostrea gigas and diet control of insulin pathway. General and Comparative Endocrinology, 176, 28 - 38. <10.1016/j.ygcen.2011.12.008>. <hal-04970709>.

2008

ref_biblio
Berthelin, C., Franco, A., Goux, D., Sourdaine, P., & Mathieu, M. (2008). Fine structure of the early stages of spermatogenesis in the Pacific oyster Crassostrea gias (Mollusca, Bivalvia). Tissue and Cell, 40 (4), 251-260. <hal-04977938>.

2003

ref_biblio
Berthelin, C., Fievet, B., Leclerc, G., Germain, P., Kellner, K., & Mathieu, M. (2003, Dec). In vivo and in vitro approaches to the analysis of glycogen metabolism in the Pacific oyster Crassostrea gigas. Journal of Shellfish Research, 22 (3), 715-720. <hal-04977933>.
ref_biblio
Berthelin, C., Kellner, K., & Mathieu, M. (2003). Transport du glucose dans les cellules vésiculeuses d'huître creuse Crassostrea gigas. Haliotis, 32, 31-40. <hal-04977760>.

2002

ref_biblio
Berthelin, C., Kellner, K., & Mathieu, M. (2002). Immunocytochemical demonstration of glucagon-like peptides in Mytilus edulis cerebral ganglia and in vitro effect of vertebrate glucagon on glycogen metabolism. Tissue and Cell, 34 (2), 109-116. <hal-04977756>.

2001

ref_biblio
Berthelin, C., Joachim, E., Juliette, L., Martinez, O., G, H., Mathieu, M., & Kellner, K. (2001). Storage and reproductive strategy in Crassostrea gigas of two different growing area (Normandy and Atlantic shore, France). Invertebrate Reproduction and Development. <hal-04977750>.

2000

ref_biblio
Berthelin, C., Kellner, K., & Mathieu, M. (2000). Histological characterization and glucose incorporation into glycogen of the Pacific oyster Crassostrea gigas. Marine Biotechnology, 2, 136-145. <hal-04971123>.
ref_biblio
Berthelin, C., Kellner, K., & Mathieu, M. (2000). Storage metabolism in the Pacific oyster (Crassostrea gigas) in relation to summer mortalities and reproductive cycle (West coast of France). Comparative Biochemistry and Physiology. Part B, Biochemistry and Molecular Biology, 125, 359-369. <hal-04977696>.

Communication dans un congrès

2025

ref_biblio
Arredondo-espinoza Roberto, C., Clairet, N., Bouillet, A., Auger, H., Kellner, K., Elie, N., Goux, D., Berthelin, C., Ordanoska, D., & Rivière, G. (2025, May). DNA methylation drives environmentally-induced gene expression by shaping chromatin structure in the oyster Crassostrea gigas. Paper presented at the EPIMAR2025 - Third International Symposium on Epigenetics in Marine and Aquatic Research, Barcelone, Spain. <hal-05605023>.
ref_biblio
Berthelin, C., Lévêque, L., Mary, V., Midroit, A., Vilain-naud, N., Elie, N., Goux, D., & Kellner, K. (2025, Jun). First isolation and characterization of EVs from the hemocytes and digestive cells of the oyster Magallana gigas (Crassostrea gigas). Paper presented at the FSEV 2025, Paris, France. <hal-05567119>. PDF
ref_biblio
Berthelin, C., Lola, L., Mary, V., Midroit, A., Vilain-naud, N., Elie, N., Goux, D., & Kellner, K. (2025). Isolation and characterization of EVs in the hemocytes and digestive cells of the oyster Crassostrea gigas. Paper presented at the Société française des vésicules extracellulaires, Paris, France. <hal-05588004>.

2023

ref_biblio
Sauger, C., Kellner, K., Berthelin, C., Elie, N., Dubroca, L., & Meleder, A.L. (2023, Sep). L'histologie, la stéréologie et l'IA : une histoire de maturité. Paper presented at the 3rd international congres of aquatic sciences - Archipel development, Saint Pierre et Miquelon, France. <hal-05567008>.
ref_biblio
Sauger, C., Kellner, K., Berthelin, C., Martin, V., Le Meleder, A., Elie, N., & Dubroca, L. (2023, Sep). Maturity and stereology relationship status : it's complicated. Paper presented at the ICES Annual Science Conference, Coppenhagen, Denmark. <hal-05567026>.

2021

ref_biblio
Sauger, C., T, C., Kellner, K., Berthelin, C., Vilain-naud, N., Elie, N., & Dubroca, L. (2021). Turning a time-consuming process into a few coded-lines: how deep learning can shorten histology slide readings for maturity staging. Paper presented at the Word Fisheries Congress, Adelaide, Australia. <hal-05578196>.

2016

ref_biblio
Akcha, F., Barranger, A., Bachère, E., Benabdelmouna, A., Rouxel, J., Rondon Sallan, R., Cosseau, C., Grunau, C., Berthelin, C., Piquemal, D., Menard, D., Montagnani, C., & Burgeot, T. (2016). Genetic and epigenetic modifications following parental exposure of the Pacific oyster Crassostrea gigas, to the herbicide diuron: the GIMEPEC project. Paper presented at the SETAC, Nantes, France. <hal-05578119>.
ref_biblio
Cherif-feildel, M., Kellner, K., Lelong, C., Goux, D., Adeline, B., & Berthelin, C. (2016, Jun). Germinal niche in an alternative hermaphrodite, the Pacific oyster C.gigas. Paper presented at the European testis workshop, Saint Malo, France. <hal-05578178>.
ref_biblio
Adeline, B., E, D., Quinquis, J., Elie, N., Kellner, K., & Berthelin, C. (2016). Comment une approche histologique couplée à une analyse quantitative peut-elle apporter des informations sur le cycle de reproduction de la sole (Solea solea) femelle?. Paper presented at the Congrès de l'Association française d'histotechnologie, Paris, France. <hal-05578167>.

2015

ref_biblio
Berthelin, C., Barranger, A., Adeline, B., Goux, D., Kellner, K., Rouxel, J., Burgeot, T., Lelong, C., & Akcha, F. (2015, Oct). Characterisation of the early germinal niche in the oyster C.gigas in the context of oxydative DNA damage in germinal cells caused by parental exposure to the herbicide diuron. Paper presented at the Congress of European society of Aquaculture, Rotterdam, Netherlands. <hal-05578155>.
ref_biblio
Lelong, C., Adeline, B., Rivière, G., Dheilly, N.M., Jouaux, A., Favrel, P., Boudry, P., Berthelin, C., & Kellner, K. (2015, Oct). Gametogenesis of triploid pacific oyster Crassostrea gigas. Paper presented at the Congress of European society of Aquaculture, Rotterdam (NL), France. <hal-05578145>.

Autre publication

2024

ref_biblio
Kellner, K., Berthelin, C., Sauger, C., Dubroca, L., Meleder, A.L., & Martin, V. (2024, Aug). Lexicon of histological structures found in the ovaries and during the oogenesis of the Striped red mullet, Mullus surmuletus, Linnaeus, 1758. <10.13155/75174>. <hal-04732935>. PDF
ref_biblio
Kellner, K., Berthelin, C., & Sauger, C. (2024). Lexicon of histological structures found in the ovaries and during the oogenesis of the four-spot megrim, Lepidorhombus boscii (Risso, 1810). <10.13155/74844>. <hal-04732944>. PDF
ref_biblio
Kellner, K., Sauger, C., & Berthelin, C. (2024). Lexicon of histological structures found in the ovaries and during the oogenesis of the megrim, Lepidorhombus whiffiagonis (Walbaum, 1792). <10.13155/75170>. <hal-04732902>. PDF
ref_biblio
Sauger, C., Le Meleder, A., Kellner, K., Berthelin, C.H., Villain-naud, N., Elie, N., & Dubroca, L. (2024). Macroscopic, histological and stereological image dataset of Four-Spot megrim (Lepidorhombus boscii) ovaries from the ICES Celtic Seas and Bay of Biscay Ecoregions. <10.5281/zenodo.13378541>. <hal-04974989>.

2023

ref_biblio
Sauger, C., Le Meleder, A., Kellner, K., Berthelin, C.H., Villain-naud, N., Elie, N., & Dubroca, L. (2023). Macroscopic, histological and stereological image dataset of Megrim (Lepidorhombus whiffiagonis) ovaries from the ICES Celtic Seas, south of Greater North sea or Bay of Biscay Ecoregions. <10.5281/zenodo.10040995>. <hal-04974985>.
ref_biblio
Sauger, C., Kellner, K., Le Meleder, A., Martin, V., Berthelin, C., Villain-naud, N., Elie, N., & Dubroca, L. (2023). Macroscopic, histological and stereological image dataset of the Striped red mullet (Mullus surmuletus) ovaries from the English Channel (ICES area 27.7.d) stock. <10.5281/zenodo.8060356>. <hal-04974981>.