• Main Research Theme:

Study of the skin microbiome of the common cuttlefish (Sepia officinalis)

Previously: Cloning and characterization of G protein-coupled receptors (GPCRs) and search for their endogenous ligand in the Pacific oyster ($Crassostrea$ $gigas$).

• Secondary Research Themes:

Contribution to other research topics within the PHARE team, depending on project needs.

Tools & Techniques

• Molecular Biology Techniques:
  DNA and RNA extraction, PCR, real-time quantitative PCR, electrophoresis, subcloning, plasmid purification, metabarcoding, Nanopore sequencing
• Bioinformatics Techniques:
  Analysis of metabarcoding data, bacterial genome assembly
• Bacteriology Techniques:
  Bacterial culture, strain maintenance, Bioscreen testing, bacteriocin testing
• Cell Culture Techniques:
  Primary culture, cell line maintenance, transfection
• Biochemical Techniques:
  Extraction of cellular components (proteins, lipids), reverse endocrinology, biochemical assays using spectrophotometer and luminometer
• Histological Techniques:
  Sample preparation, microtome sectioning, cryostat sectioning, staining, immunohistochemistry, in situ hybridization, laser microdissection