My research focuses on reproductive physiology and the management of energy reserves in several marine species, primarily mollusks.

Reproductive Studies in Marine Species

I have studied gametogenesis in the Pacific oyster (Crassostrea gigas), both in diploid and triploid individuals. Our work demonstrated that some triploid oysters are capable of producing gametes, challenging the assumption of their sterility.

I have also contributed to the qualitative and quantitative assessment of sexual maturity in various fish species, including Solea solea (common sole), Pleuronectes platessa (European plaice), Lepidorhombus boscii (four-spot megrim), Lepidorhombus whiffiagonis (megrim), and Mullus surmuletus (striped red mullet). For this, I developed a stereological approach based on histological image analysis of gonad sections. The data obtained for megrim were used to build a deep learning model to classify maturity stages.

I am also investigating the reproductive biology of the gastropod Buccinum undatum (common whelk), a flagship species along the Normandy coast. Climate change has raised serious concerns for this fishery, which is currently under pressure. We aim to identify the most climate-sensitive stages of the whelk’s life cycle.

Joint Regulation of Glycogen Storage and Reproduction

In Crassostrea gigas, glycogen reserves are essential for reproductive success. We have characterized vesicular cells, which specialize in glycogen storage, from both histological and metabolic perspectives. Controlled conditioning experiments have allowed us to study the impact of these reserves on reproductive output and gamete quality.

Characterization and Role of Insulin-like Molecules

The insulin signaling pathway plays a role in many physiological processes in Crassostrea gigas. We have identified the sequences of several insulin-like ligands and studied their expression throughout the annual cycle using in situ hybridization. These ligands are part of complex regulatory mechanisms involving the neurosecretory system.

Intercellular Communication via Extracellular Vesicles

A recent axis of my research investigates the role of extracellular vesicles in cell-to-cell communication in Crassostrea gigas.

Techniques and Approaches

My work combines cellular techniques (light, confocal and electron microscopy; quantitative histo-imaging; cell isolation and culture; laser microdissection; immunological methods) and molecular biology (proteomics, 2D electrophoresis, transcriptomics, in situ hybridization). I also conduct in vivo studies, both in the field and under controlled laboratory conditions.